- Alverno College * (Milwaukee, Wis.)
- Aurora University * (Aurora, Ill.)
- Benedictine University * (Lisle, Ill.)
- Concordia University Chicago * (River Forest, Ill.)
- Concordia University Wisconsin * (Mequon, Wis.)
- Dominican University * (River Forest, Ill.)
- Edgewood University * (Madison, Wis.)
- Illinois Institute of Technology # (Chicago, Ill.)
- Lakeland University * (Plymouth, Wis.)
- Marian University * (Fond du Lac, Wis.)
- Milwaukee School of Engineering ^ (Milwaukee, Wis.)
- Rockford University * (Rockford, Ill.)
- St. Norbert College $ (De Pere, Wis.)
- Wisconsin Lutheran College * (Milwaukee, Wis.)
* - charter member
^ - joined NACC Fall 2007
# - joined NACC Fall 2018
$ - joined NACC Fall 2019 as a Golf/MVB affiliate, Fall 2021 as a full member
Former Full Members
- Maranatha Baptist Bible College, Watertown, Wis. (2006-2013, charter member)
Affiliate Members
- Beloit College (Beloit, Wis.) -- Men's Lacrosse/Women's Lacrosse (Spring 2021-present)
- Cornell College (Mount Vernon, Iowa) -- Men's Lacrosse/Women's Lacrosse (Spring 2025-present)
- University of Dubuque (Dubuque, Iowa) -- Men's Lacrosse (Spring 2025-present)
- Eureka College (Eureka, Ill.) -- Football (Fall 2018-present)
- Lake Forest College (Lake Forest, Ill.) -- Men's Lacrosse/Women's Lacrosse (Spring 2025-present)
- Lawrence University (Appleton, Wis.) -- Men's Lacrosse/Women's Lacrosse (Spring 2025-present)
Former Affiliate Members