• Aurora University * (Aurora, Ill.)
• Benedictine University * (Lisle, Ill.)
• Concordia University Chicago * (River Forest, Ill.)
• Concordia University Wisconsin * (Mequon, Wis.)
• Dominican University * (River Forest, Ill.)
• Edgewood University * (Madison, Wis.)
• Illinois Institute of Technology # (Chicago, Ill.)
• Lakeland University * (Plymouth, Wis.)
• Marian University * (Fond du Lac, Wis.)
• Milwaukee School of Engineering ^ (Milwaukee, Wis.)
• Rockford University * (Rockford, Ill.)
• St. Norbert College $ (De Pere, Wis.)
• Wisconsin Lutheran College * (Milwaukee, Wis.)

* - charter member
^ - joined NACC Fall 2007
# - joined NACC Fall 2018
$ - joined NACC Fall 2019 as a Golf/MVB affiliate, Fall 2021 as a full member

Former Full Members

• Alverno College, Milwaukee, Wis. (2006-2026, charter member)
• Maranatha Baptist Bible College, Watertown, Wis. (2006-2013, charter member)

Affiliate Members

• Alverno College (Milwaukee, Wis.) -- Women's Golf (Fall 2026-present)
• Beloit College (Beloit, Wis.) -- Men's Lacrosse/Women's Lacrosse (Spring 2021-present)
• Cornell College (Mount Vernon, Iowa) -- Men's Lacrosse/Women's Lacrosse (Spring 2025-present)
• University of Dubuque (Dubuque, Iowa) -- Men's Lacrosse (Spring 2025-present)
• Eureka College (Eureka, Ill.) -- Football (Fall 2018-present)
• Lake Forest College (Lake Forest, Ill.) -- Men's Lacrosse/Women's Lacrosse (Spring 2025-present); Men's Volleyball (Spring 2027-present)
• Lawrence University (Appleton, Wis.) -- Men's Lacrosse/Women's Lacrosse (Spring 2025-present)

Former Affiliate Members

• Mount Mary University (Milwaukee, Wis.) -- Women's Tennis (2015-16); Women's Cross Country (2015-2024)